Enzymes and Inhibitors
Sigma Aldrich Fine Chemicals Biosciences Lipase from Candida rugosa Type VII, ≥700 unit/mg solid | 9001-62-1 | MFCD00131509 | 100g
Lipase from Candida rugosa Type VII, ≥700 unit/mg solid | 9001-62-1 | MFCD00131509 | 100g
Worthington Biochemical Corporation PURIFIED HYALURONIDASE 15KU
Chromatographically purified. A dialyzed, lyophilized powder.
Zymo Research Corporation DNA Degradase Plus™ (250 U)
DNA Degradase Plus from Zymo Research is a nuclease mix that quickly and efficiently degrades DNA to its individual nucleoside components. Since nucleosides lack negatively charged phosphate, DNA Degradase Plus is ideal for whole-genome DNA methylation analysis by LC/MS. Digestion with the enzyme is performed via a one-step procedure that is faster and simpler than other available methods.
Sigma Aldrich Fine Chemicals Biosciences Phosphatase, Alkaline from bovine intestinal mucosa >=2,000 DEA units/mg protein | 9001-78-9 | MFCD00131849 | 10KU
Phosphatase, Alkaline from bovine intestinal mucosa >=2,000 DEA units/mg protein | 9001-78-9 | MFCD00131849 | 10KU
Sigma Aldrich Fine Chemicals Biosciences Phosphatase, Alkaline from bovine intestinal mucosa, buffered aqueous glycerol solution, ≥5,500 DEA units/mg protein | 9001-78-9 | MFCD00131849 | 50ku
Phosphatase, Alkaline from bovine intestinal mucosa, buffered aqueous glycerol solution, ≥5,500 DEA units/mg protein | 9001-78-9 | MFCD00131849 | 50ku
LGC GENOMICS LLC (Lucigen) Rec J Exonuclease, 250 U @ 10 U/LMagnesium-dependent exonuclease for 5' to 3' digestion of single-stranded DNA.
Rec J Exonuclease, derived from E. coli, catalyzes degradation of ssDNA in a 53 direction. Its activity is dependent on Mg2+. Rec J Exonuclease can be heat-inactivated by incubation at 65C for 20 minutes.
Enzo Life Sciences MMP-14 (catalytic domain) (human), (recombinant) (10 µg)
Alternative name: Matrix metalloproteinase 14. Membrane type matrix metalloproteinase 1. MT-1 MMP. MW: 22.5 kDa. Formulation: Liquid. In 50mM TRIS, pH 7.5, containing 5mM calcium chloride, 300mM sodium chloride, 20µM zinc chloride, 0.5% Brij-35, and 30% glycerol. Source: Produced in E.coli. MMP-14, Membrane-Type Matrix Metalloproteinase 1, MT1-MMP catalytic domain from human cDNA. The enzyme consists of the catalytic domain of human MMP-14 (Tyr112-Arg298, NM_004995) with a C-terminal purification tag. This represents a naturally-occurring active form of MMP-14 which lacks the C-terminal hemopexin domain. MMPs lacking this domain cannot cleave native collagens; however, activity toward other targets such as gelatin. CASein, or peptide substrates is unaffected. Use/Stability: The enzyme is stable on ice for at least several hours. However, it is recommended that thawing and dilution of the enzyme be done within as short a time as possible before start of the assay.
New England Biolabs, Inc. SplintR Ligase – 1250 units
SplintR Ligase efficiently catalyzes the ligation of adjacent, single-stranded DNA splinted by a complementary RNA strand. This activity may enable novel approaches for characterization of miRNAs and mRNAs, including SNPs. The robust activity of the enzyme and its affinity for RNA-splinted DNA substrates (apparent Km = 1 nM) enable sub-nanomolar detection of unique RNA species within a complex mixture, making SplintR ligase a superior choice for demanding RNA detection technologies. The activity is enhanced at higher temperatures (up to 37°C), and by supplementation with 5 mM Mn2+. The reaction is inhibited by salt concentrations in excess of 100 mM. The enzyme tolerates all base pair combinations at the ligation junction, but is partially inhibited by dC/G and dG/C base pairs at the donor (phosphorylated) side ligation junction, particularly when the +2 base was also a C/G base pair.
MilliporeSigma TMB/E SINGLE REAGENT
TMB/E Single Reagent, Blue color, Horseradish Peroxidase Substrate (soluble)
![Research Products International Corp PHENYL-B-D-GALACTOPYRANOSIDE [P-GAL], 5 GRAM](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-P12000-5.02.JPG-250.jpg)
Research Products International Corp PHENYL-B-D-GALACTOPYRANOSIDE [P-GAL], 5 GRAM
- Substance Name: Phenyl-β-D-galactopyranoside
- CAS Number: 2818-58-8
- Molecular Formula: C12H16O6
- Molecular Weight: 256.26
New England Biolabs, Inc. HincII – 1000 units
A restriction endonuclease that recognizes the sequence GTY_^RAC.
New England Biolabs, Inc. ShortCut RNase III – 200 units
ShortCut RNase III, used with its manganese-containing reaction buffer, converts long double-stranded RNA into a heterogeneous mix of short (18-25 bp) interfering RNAs (siRNA) suitable for RNA interference in mammalian cells. 1.5 units (1 µl) of ShortCut RNase III is sufficient to convert 1µ g of dsRNA into siRNA suitable for RNA interference in mammalian cells.
New England Biolabs, Inc. Bst DNA Polymerase, Large Fragment – 8000 units
Bst DNA Polymerase, Large Fragment is the portion of the Bacillus stearothermophilus DNA Polymerase protein that contains the 5' to 3' polymerase activity, but lacks 5' to 3' exonuclease activity.
New England Biolabs, Inc. HaeIII – 3000 units
A restriction endonuclease that recognizes the sequence GG_^CC.
New England Biolabs, Inc. Hpy188I – 1000 units
A restriction endonuclease that recognizes the sequence TC_N^GA.